polyclonal anti-lysozyme 1 rabbit primary antibody Search Results


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Thermo Fisher polyclonal anti-lysozyme 1 rabbit primary antibody
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Agilent technologies lyz1-rabbit polyclonal anti-lysozyme (1:2000
Antibiotics in early life lead to higher number of Paneth cells and few changes in enteroendocrine markers in the proximal small intestine. ( A ) Whole tissue real-time qPCR analysis of adult brush-border enzymes Sis and Arg2 and of Paneth cell marker <t>Lyz1</t> in proximal small intestine. Relative expression to reference genes Rpl4 and Ppib. Sis: n = 11 control mice, n = 12 AB-treated mice; Arg2: n = 11 control mice, n = 11 AB-treated mice; Lyz1: n = 11 control mice, n = 10 AB-treated mice. ( B ) Immunohistochemistry of <t>lysozyme-1</t> in proximal small intestine, n = 11 control mice, n = 12 AB-treated mice. (Scale bars, 20 µm.) ( C ) Whole tissue real-time qPCR analysis of adult brush-border enzymes Sis and Arg2 and of Paneth cell marker Lyz1 in distal small intestine. Relative expression to reference genes Rpl4 and Ppib. Sis: n = 11 control mice, n = 10 AB-treated mice; Arg2: n = 10 control mice, n = 9 AB-treated mice; Lyz1: n = 11 control mice, n = 10 AB-treated mice. ( D ) Immunohistochemistry of lysozyme-1 in distal small intestine, n = 10 control mice, n = 12 AB-treated mice (Scale bars, 20 µm.) ( E ) Whole tissue real-time qPCR analysis of enteroendocrine markers Sct, Cck, Gip, Sst, Gcg, and Pyy in proximal small intestine. Relative expression to reference genes Rpl4 and Ppib. Sct, Cck, Gip, Sst: n = 11 control mice, n = 12 AB-treated mice. Gcg: n = 11 control mice, n = 10 AB-treated mice. Pyy: n = 11 control mice, n = 11 AB-treated mice. Statistical analysis was performed by one-tailed unpaired t -test or by Mann–Whitney test when data were not normally distributed as assessed by D’Agostino and Pearson normality test (Sct). Error bars indicate the mean ± SD. Levels of significance are indicated (* p < 0.05, ** p < 0.01, *** p < 0.005).
Lyz1 Rabbit Polyclonal Anti Lysozyme (1:2000, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Antibiotics in early life lead to higher number of Paneth cells and few changes in enteroendocrine markers in the proximal small intestine. ( A ) Whole tissue real-time qPCR analysis of adult brush-border enzymes Sis and Arg2 and of Paneth cell marker Lyz1 in proximal small intestine. Relative expression to reference genes Rpl4 and Ppib. Sis: n = 11 control mice, n = 12 AB-treated mice; Arg2: n = 11 control mice, n = 11 AB-treated mice; Lyz1: n = 11 control mice, n = 10 AB-treated mice. ( B ) Immunohistochemistry of lysozyme-1 in proximal small intestine, n = 11 control mice, n = 12 AB-treated mice. (Scale bars, 20 µm.) ( C ) Whole tissue real-time qPCR analysis of adult brush-border enzymes Sis and Arg2 and of Paneth cell marker Lyz1 in distal small intestine. Relative expression to reference genes Rpl4 and Ppib. Sis: n = 11 control mice, n = 10 AB-treated mice; Arg2: n = 10 control mice, n = 9 AB-treated mice; Lyz1: n = 11 control mice, n = 10 AB-treated mice. ( D ) Immunohistochemistry of lysozyme-1 in distal small intestine, n = 10 control mice, n = 12 AB-treated mice (Scale bars, 20 µm.) ( E ) Whole tissue real-time qPCR analysis of enteroendocrine markers Sct, Cck, Gip, Sst, Gcg, and Pyy in proximal small intestine. Relative expression to reference genes Rpl4 and Ppib. Sct, Cck, Gip, Sst: n = 11 control mice, n = 12 AB-treated mice. Gcg: n = 11 control mice, n = 10 AB-treated mice. Pyy: n = 11 control mice, n = 11 AB-treated mice. Statistical analysis was performed by one-tailed unpaired t -test or by Mann–Whitney test when data were not normally distributed as assessed by D’Agostino and Pearson normality test (Sct). Error bars indicate the mean ± SD. Levels of significance are indicated (* p < 0.05, ** p < 0.01, *** p < 0.005).

Journal: Antibiotics

Article Title: Altered Gut Structure and Anti-Bacterial Defense in Adult Mice Treated with Antibiotics during Early Life

doi: 10.3390/antibiotics11020267

Figure Lengend Snippet: Antibiotics in early life lead to higher number of Paneth cells and few changes in enteroendocrine markers in the proximal small intestine. ( A ) Whole tissue real-time qPCR analysis of adult brush-border enzymes Sis and Arg2 and of Paneth cell marker Lyz1 in proximal small intestine. Relative expression to reference genes Rpl4 and Ppib. Sis: n = 11 control mice, n = 12 AB-treated mice; Arg2: n = 11 control mice, n = 11 AB-treated mice; Lyz1: n = 11 control mice, n = 10 AB-treated mice. ( B ) Immunohistochemistry of lysozyme-1 in proximal small intestine, n = 11 control mice, n = 12 AB-treated mice. (Scale bars, 20 µm.) ( C ) Whole tissue real-time qPCR analysis of adult brush-border enzymes Sis and Arg2 and of Paneth cell marker Lyz1 in distal small intestine. Relative expression to reference genes Rpl4 and Ppib. Sis: n = 11 control mice, n = 10 AB-treated mice; Arg2: n = 10 control mice, n = 9 AB-treated mice; Lyz1: n = 11 control mice, n = 10 AB-treated mice. ( D ) Immunohistochemistry of lysozyme-1 in distal small intestine, n = 10 control mice, n = 12 AB-treated mice (Scale bars, 20 µm.) ( E ) Whole tissue real-time qPCR analysis of enteroendocrine markers Sct, Cck, Gip, Sst, Gcg, and Pyy in proximal small intestine. Relative expression to reference genes Rpl4 and Ppib. Sct, Cck, Gip, Sst: n = 11 control mice, n = 12 AB-treated mice. Gcg: n = 11 control mice, n = 10 AB-treated mice. Pyy: n = 11 control mice, n = 11 AB-treated mice. Statistical analysis was performed by one-tailed unpaired t -test or by Mann–Whitney test when data were not normally distributed as assessed by D’Agostino and Pearson normality test (Sct). Error bars indicate the mean ± SD. Levels of significance are indicated (* p < 0.05, ** p < 0.01, *** p < 0.005).

Article Snippet: LYZ1-rabbit polyclonal anti-lysozyme (1:2000, DAKO, A0099).

Techniques: Marker, Expressing, Immunohistochemistry, One-tailed Test, MANN-WHITNEY